Method for Selection of Mutagen-sensitive Mutants ofEscherichia coli
نویسندگان
چکیده
منابع مشابه
Third-chromosome mutagen-sensitive mutants of Drosophila melanogaster.
A total of 34 third chromosomes of Drosophila melanogaster that render homozygous larvae hypersensitive to killing by chemical mutagens have been isolated. Genetic analyses have placed responsible mutations in more than eleven complementation groups. Mutants in three complementation groups are strongly sensitive to methyl methanesulfonate, those in one are sensitive to nitrogen mustard, and mut...
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Mutants of Escherichia coli sensitive to the antibiotic synergistin A, an inhibitor of protein synthesis, were isolated. These mutants were pleiotropic, being also sensitive to a large number of unrelated antibiotics and to lysis by detergents. These pleiotropic responses indicated that the mutations affected cell wall or membrane synthesis. Consequently, selection for antibiotic-sensitive muta...
متن کاملSelection of mutants of Escherichia coli constitutive for tryptophanase.
Proc. Natl. Acad. Sci. U.S. 40:1064, 1954). The galactose mutations are of the same stability as the streptomycin-sensitivity gene. Some 60 other galactose mutants are similar to those reported in Table 1. No instances of mutability grossly higher than Gal16 or Galg have been noted, and no certain cases of failure to revert have been found. Combinations of Gal mutations have been synthesized vi...
متن کاملThe effects of mutagen-sensitive mutants of Drosophila melanogaster in nonmutagenized cells.
The effects of 13 mutagen-sensitive (mus) mutants (representing seven loci) on mitotic chromosome stability in nonmutagenized cells have been examined genetically. To do this, mus-bearing flies heterozygous for the recessive somatic-cell marker, multiple wing hairs (mwh), were examined for increased frequencies of mwh clones in the wing blade. Mutants at the mus-103, mus-104 and mus-106 loci do...
متن کاملThe isolation of mutagen-sensitive nuv mutants of Aspergillus nidulans and their effects on mitotic recombination.
More than 200 mutants of Aspergillus nidulans were isolated as hypersensitive to the monofunctional alkylating agent MNNG and/or UV-irradiation (designated nuv mutants). Of these, 23 were selected for further characterization. All were markedly hypersensitive to both MNNG and the quasi-UV-mimetic mutagen 4-NQO. The hypersensitive phenotype of each mutant was shown to result from mutation of a s...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1977
ISSN: 0002-1369
DOI: 10.1080/00021369.1977.10862887